P-59: Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging,Miscarriage and Pregnancy Rate in Infertile Men with Varicocele

Background: Although the pathophysiology of the testicular damage associated with varicocele remains unclear, but varicocelectomy is considered a suitable technique for the treatment of individuals with clinical varicocele. The current study was designed to assessed semen parameters, chromatin packaging, and pregnancy outcome before and after varicocelectomy. Materials and Methods: Semen samples were obtained from men with varicocele grades II and III (n = 145). Samples were assessed by a spermiogram, and chromatin packaging (chromomycin A3), before and after surgery microsurgical varicocelectomy was performed as part of patient management. Data was analyzed by SPSS. 11.5. Results: Sperm parameters and chromatin packaging were improved after surgery compared to before surgery. The percentages of spontaneous cumulative pregnancies were 33.1, 42.06, 46.2, 48.9, and 55.8% after 3, 6, 9, 12 months post-surgery respectively. In addition, percentages of spontaneous cumulative miscarriage were 2.46, 4.93, 4.93, 6.17, and 6.17 % after 3, 6, 9, 12 months postsurgery respectively. Conclusion: Microsurgical varicocelectomy in addition to improves sperm parameters and chromatin packaging, also improve the chance of pregnancy.